Journal: PLOS One
Article Title: Circular RNA FTO functions as a hsa-miR-141-3p sponge to regulate the growth and migration abilities of human retinal endothelial cells via up-regulating ZEB1
doi: 10.1371/journal.pone.0338208
Figure Lengend Snippet: A-B: The target hsa-miR-141-3p and binding sites of ZEB1 was confirmed by the targetscan database and validated by the dual-luciferase reporter gene system. The ZEB1 expressions in HRECs were measured with RT-qPCR after hsa-miR-141-3p mimic (C). The mRNA (D) and protein (E) levels of ZEB1 were detected by RT-qPCR and western blot in HG-treated and circFTO knockdown HRECs. (A-C) The differences were detected using Student-T test (n=3). (D) The differences were detected using One-way ANOVA (n=3).
Article Snippet: Human retinal microvascular endothelial cells (HRECs) were obtained from Innoprot® (P10880; Derio–Bizkaia, Spain).
Techniques: Binding Assay, Luciferase, Quantitative RT-PCR, Western Blot, Knockdown